[3H]Phorbol 12,13-dibutyrate binding assay for protein kinase C and related proteins

Methods Mol Biol. 2003:233:129-56. doi: 10.1385/1-59259-397-6:129.

Authors

Nancy E Lewin¹, Peter M Blumberg

Affiliation

¹ Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

PMID: 12840504
DOI: 10.1385/1-59259-397-6:129

No abstract available

MeSH terms

Ligands
Phorbol 12,13-Dibutyrate / chemistry
Phorbol 12,13-Dibutyrate / metabolism*
Protein Binding
Protein Kinase C / metabolism*
Radioligand Assay / methods*
Tritium / metabolism*

Substances

Ligands
Tritium
Phorbol 12,13-Dibutyrate
Protein Kinase C