Abstract
A cellular biosensing system has been constructed to assess the biological safety/toxicity of chemicals. Detection of nitric oxide (NO) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals, because some are known to induce NO synthase (iNOS) activity thereby increasing NO production. The macrophage-like cell line, RAW264.7, was cultured on the electrode coated with a polyion complex layer. The potent immune activating abilities of lipopolysaccharide could be verified by the cellular biosensing system: NO release from cells was detected within 600 ms by double potential step chronoamperometry.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Adjuvants, Immunologic / analysis
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Animals
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Biosensing Techniques / instrumentation*
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Biosensing Techniques / methods*
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Cells, Cultured
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Electrochemistry / instrumentation
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Electrochemistry / methods
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Equipment Design
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Nitric Oxide / metabolism
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Nitric Oxide Synthase / metabolism
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Nitric Oxide Synthase Type II
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Nitrous Oxide / metabolism
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Reproducibility of Results
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Sensitivity and Specificity
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Toxicity Tests / instrumentation*
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Toxicity Tests / methods
Substances
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Adjuvants, Immunologic
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Lipopolysaccharides
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Nitric Oxide
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Nitrous Oxide