Cryo-EM of the native structure of the calcium release channel/ryanodine receptor from sarcoplasmic reticulum

Biophys J. 1992 Apr;61(4):936-40. doi: 10.1016/S0006-3495(92)81900-8.

Abstract

The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water and in negative stained specimens. Features seen in the three-dimensional reconstruction of the negatively stained channel can be identified in the projection of the unstained receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biophysical Phenomena
  • Biophysics
  • Calcium Channels / ultrastructure*
  • Freezing
  • Microscopy, Electron
  • Muscle Proteins / ultrastructure*
  • Protein Conformation
  • Rabbits
  • Receptors, Cholinergic / ultrastructure*
  • Ryanodine Receptor Calcium Release Channel
  • Sarcoplasmic Reticulum / ultrastructure

Substances

  • Calcium Channels
  • Muscle Proteins
  • Receptors, Cholinergic
  • Ryanodine Receptor Calcium Release Channel