Abstract
Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor. A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1 beta to the mature form. Sequence analysis indicated that the enzyme itself may undergo proteolytic processing. The gene encoding the protease was mapped to chromosomal band 11q23, a site frequently involved in rearrangement in human cancers.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Caspase 1
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Cell Line
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Chromosome Banding
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Chromosomes, Human, Pair 11*
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Cloning, Molecular
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Enzyme Precursors / biosynthesis
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Enzyme Precursors / genetics*
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Enzyme Precursors / isolation & purification
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Humans
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Metalloendopeptidases / biosynthesis
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Metalloendopeptidases / genetics*
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Metalloendopeptidases / isolation & purification
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Molecular Sequence Data
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Neutrophils / enzymology
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Oligodeoxyribonucleotides
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Poly A / genetics
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Poly A / isolation & purification
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Polymerase Chain Reaction / methods
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger / genetics
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / isolation & purification
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Transfection
Substances
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Enzyme Precursors
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Oligodeoxyribonucleotides
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RNA, Messenger
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Recombinant Proteins
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Poly A
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RNA
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Caspase 1
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Metalloendopeptidases