Species heterogeneity of hepatic alpha 1-adrenoceptors: alpha 1A-, alpha 1B- and alpha 1C-subtypes

Biochem Biophys Res Commun. 1992 Jul 31;186(2):760-7. doi: 10.1016/0006-291x(92)90811-x.

Abstract

alpha 1-Adrenergic activation stimulated phosphorylase and phosphoinositide turnover in hepatocytes from guinea pigs, rats and rabbits. Chlorethylclonidine inhibited these effects in rat and rabbit cells but not in guinea pig hepatocytes; low concentrations of 5-methyl urapidil blocked the alpha 1 actions in guinea pig and rabbit liver cells, but not in rat hepatocytes. Binding competition experiments also showed high affinity for 5-methyl urapidil in liver membranes from guinea pigs and rabbits and low affinity in those from rats. The data indicated that guinea pig hepatocytes express alpha 1A-, rat hepatocytes alpha 1B- and rabbit hepatocytes alpha 1C- adrenoceptors. This was confirmed by Northern analysis using receptor subtype-selective probes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-Antagonists / pharmacology
  • Animals
  • Blotting, Northern
  • Cells, Cultured
  • Guinea Pigs
  • Inositol 1,4,5-Trisphosphate / metabolism*
  • Kinetics
  • Liver / drug effects
  • Liver / metabolism*
  • Norepinephrine / pharmacology
  • Piperazines / pharmacology
  • Prazosin / pharmacology
  • Propranolol / pharmacology
  • RNA / genetics
  • RNA / isolation & purification
  • Rabbits
  • Rats
  • Rats, Inbred Strains
  • Receptors, Adrenergic, alpha / drug effects
  • Receptors, Adrenergic, alpha / genetics
  • Receptors, Adrenergic, alpha / physiology*
  • Species Specificity

Substances

  • Adrenergic alpha-Antagonists
  • Piperazines
  • Receptors, Adrenergic, alpha
  • 5-methylurapidil
  • RNA
  • Inositol 1,4,5-Trisphosphate
  • Propranolol
  • Norepinephrine
  • Prazosin