Abstract
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
MeSH terms
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Animals
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Antibodies / chemistry*
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Antibodies, Monoclonal / metabolism
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Antigens / metabolism
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Blotting, Western
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Cell Line, Tumor
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Chromatography
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Chromatography, Gel
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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Hepatitis B Surface Antigens / isolation & purification*
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Hepatitis B Vaccines / chemistry
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Humans
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Immunoglobulin G / metabolism
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Ligands
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Mice
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Mice, Inbred BALB C
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Nicotiana / chemistry
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Nicotiana / immunology
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Plant Proteins / metabolism
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Plants / immunology*
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Plants, Genetically Modified
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Recombinant Proteins / chemistry
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Vaccines, Synthetic / chemistry
Substances
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Antibodies
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Antibodies, Monoclonal
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Antigens
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Hepatitis B Surface Antigens
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Hepatitis B Vaccines
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Immunoglobulin G
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Ligands
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Plant Proteins
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Recombinant Proteins
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Vaccines, Synthetic