Abstract
OAZ is a multi zinc finger protein which interacts with several distinct nuclear proteins including Olf-1/EBF, Smad1, and Smad4 and regulates transcription of various genes. Here we show that poly(ADP-ribose) polymerase 1 (Parp1) forms a complex with OAZ and positively regulates BMP-target genes, Xenopus Vent-2 and mouse Smad6 genes. Both wild type and the mutant forms of Parp1, which is deficient in poly(ADP-ribose) polymerase activity, constitutively interact with OAZ; however, the mutant Parp1 did not activate transcription. These results suggest that poly(ADP-ribose) polymerase activity is essential for the transcriptional activation of Vent-2 and Smad6. These results suggest that Parp1 serves as a transcriptional coactivator of OAZ in BMP-dependent gene regulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Bone Morphogenetic Proteins / metabolism
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COS Cells
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Chromatin / metabolism
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DNA-Binding Proteins / metabolism
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Enzyme Inhibitors / pharmacology
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Gene Expression Regulation, Enzymologic
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Homeodomain Proteins / metabolism
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Luciferases / metabolism
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Mice
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Mutation
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Plasmids / metabolism
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Poly(ADP-ribose) Polymerases / chemistry*
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Poly(ADP-ribose) Polymerases / metabolism
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Protein Binding
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RNA / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Smad6 Protein
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Trans-Activators / metabolism
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Transcription Factors*
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Transcriptional Activation
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Transfection
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Transforming Growth Factor beta / metabolism
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Two-Hybrid System Techniques
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Xenopus
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Xenopus Proteins*
Substances
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Bone Morphogenetic Proteins
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Chromatin
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DNA-Binding Proteins
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Enzyme Inhibitors
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Homeodomain Proteins
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Smad6 Protein
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Smad6 protein, mouse
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Trans-Activators
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Transcription Factors
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Transforming Growth Factor beta
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Xenopus Proteins
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ventx2.1 protein, Xenopus
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RNA
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Luciferases
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Poly(ADP-ribose) Polymerases