Viable cell detection by the combined use of fluorescent glucose and fluorescent glycine

Hideaki Matsuoka; Kanenari Oishi; Masaaki Watanabe; Ikuko Kozone; Mikako Saito; Shizunobu Igimi

doi:10.1271/bbb.67.2459

Viable cell detection by the combined use of fluorescent glucose and fluorescent glycine

Biosci Biotechnol Biochem. 2003 Nov;67(11):2459-62. doi: 10.1271/bbb.67.2459.

Authors

Hideaki Matsuoka¹, Kanenari Oishi, Masaaki Watanabe, Ikuko Kozone, Mikako Saito, Shizunobu Igimi

Affiliation

¹ Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan. bio-func@cc.tuat.ac.jp

PMID: 14646209
DOI: 10.1271/bbb.67.2459

Abstract

The combined use of a fluorescent glucose (2NBDG) and a fluorescent glycine (NBD-Gly) was tried for the detection of viable cells of significant foodborne pathogenic strains in addition to several Escherichia coli strains and coliforms. Thirty-five out of 41 strains showed marked uptake of 2NBDG but 6 strains were not able to take in 2NBDG. Five out of these 6 strains showed NBD-Gly uptake.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

4-Chloro-7-nitrobenzofurazan* / analogs & derivatives*
Aeromonas hydrophila / growth & development
Bacteria / growth & development*
Deoxyglucose* / analogs & derivatives*
Escherichia coli / growth & development
Fluorescent Dyes*
Glycine / analogs & derivatives*
Listeria monocytogenes / growth & development
Pseudomonas aeruginosa / growth & development
Salmonella typhimurium / growth & development

Substances

Fluorescent Dyes
Deoxyglucose
4-Chloro-7-nitrobenzofurazan
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose
Glycine