Abstract
The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to identify an isolate of S. haemolyticus fully. The PCR assay, which detects polymorphism in the 16S-23S rRNA spacer region, is shown to be potentially useful for rapid and accurate identification of coagulase-negative staphylococci. Identical PFGE type and antibiotic-resistance profiles of two methicillin-resistant S. haemolyticus isolates in this study suggest the existence of a multiresistant community clone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Child
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DNA Fingerprinting
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DNA, Bacterial / analysis
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DNA, Bacterial / chemistry
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Drug Resistance, Multiple, Bacterial
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Electrophoresis, Gel, Pulsed-Field
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Female
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Humans
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Male
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Microbial Sensitivity Tests
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Phenotype
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Soft Tissue Infections / microbiology*
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Staphylococcal Infections / microbiology*
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Staphylococcal Skin Infections / microbiology*
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Staphylococcus / classification
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Staphylococcus / drug effects
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Staphylococcus / genetics
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Staphylococcus / isolation & purification*
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Staphylococcus haemolyticus / classification
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Staphylococcus haemolyticus / drug effects
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Staphylococcus haemolyticus / genetics
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Staphylococcus haemolyticus / isolation & purification*
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Wound Infection / microbiology*