Diffusion tensor MR imaging is a technique that provides details on tIssue microstructure and organization well beyond the usual image resolution. With diffusion tensor MR imaging, diffusion anisotropy can be quantified and subtle white matter changes not normally seen on conventional MRI can be detected. The purpose of this article is to review the current applications of diffusion tensor MR imaging for the study of the brain, including normal aging, Alzheimer's disease, neuropsychiatric disorders, strokes, multiple sclerosis, brain tumors and intractable seizures.