Overproduction of eukaryotic SUMO-1- and SUMO-2-conjugated proteins in Escherichia coli

Anal Biochem. 2004 Aug 1;331(1):204-6. doi: 10.1016/j.ab.2004.04.034.

Authors

Yasuhiro Uchimura¹, Makoto Nakamura, Kaoru Sugasawa, Mitsuyoshi Nakao, Hisato Saitoh

Affiliation

¹ Department of Regeneration Medicine, Institute of Molecular Embryology Genetics, Kumamoto University, Kumamoto, 860-0811, Japan.

PMID: 15246018
DOI: 10.1016/j.ab.2004.04.034

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Escherichia coli / genetics*
Humans
Plasmids / genetics
Protein Binding / genetics
Protein Binding / physiology
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / isolation & purification
Recombinant Fusion Proteins / metabolism
SUMO-1 Protein
Small Ubiquitin-Related Modifier Proteins / genetics*
Small Ubiquitin-Related Modifier Proteins / isolation & purification
Small Ubiquitin-Related Modifier Proteins / metabolism
Ubiquitin-Conjugating Enzymes / genetics*
Ubiquitin-Conjugating Enzymes / isolation & purification
Ubiquitin-Conjugating Enzymes / metabolism

Substances

Recombinant Fusion Proteins
SUMO-1 Protein
SUMO1 protein, human
SUMO2 protein, human
Small Ubiquitin-Related Modifier Proteins
Ubiquitin-Conjugating Enzymes
ubiquitin-conjugating enzyme UBC9