Tumor necrosis factor-alpha gene is expressed in stimulated retinal pigment epithelial cells in culture

Biochem Biophys Res Commun. 1992 Sep 16;187(2):1029-34. doi: 10.1016/0006-291x(92)91300-f.

Abstract

Expression of TNF-alpha gene in the retinal pigment epithelial (RPE) cells was studied by using polymerase chain reaction (PCR). PCR for human TNF-alpha gene using complementary DNA (cDNA) of control RNA prepared from non-stimulated RPE cells failed to show expression of TNF-alpha gene. However, PCR by using cDNA prepared from interleukin-1 beta (IL-1 beta)-stimulated RPE cells revealed expression of the gene, suggesting in vivo production of TNF-alpha from RPE cells in response to IL-1 beta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / chemistry
  • DNA / genetics
  • Gene Expression*
  • Humans
  • Interleukin-1 / pharmacology
  • Kinetics
  • Molecular Sequence Data
  • Pigment Epithelium of Eye / metabolism*
  • Platelet-Derived Growth Factor / pharmacology
  • Polymerase Chain Reaction
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thrombin / pharmacology
  • Transforming Growth Factor beta / pharmacology
  • Tumor Necrosis Factor-alpha / genetics*

Substances

  • Interleukin-1
  • Platelet-Derived Growth Factor
  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha
  • DNA
  • Thrombin
  • Tetradecanoylphorbol Acetate