An agonist human ICOS monoclonal antibody that induces T cell activation and inhibits proliferation of a myeloma cell line

Hybrid Hybridomics. 2004 Jun;23(3):176-82. doi: 10.1089/1536859041224299.

Abstract

The inducible costimulator (ICOS), the third member of the CD28/CD152 receptor family, is an important costimulatory molecule during the immune response. In this study, a functional anti-human ICOS MAb 2C7 was obtained. The specificity of this MAb was verified by flow cytometry, Western blotting, and competition with anti-ICOS MAb ISA3. This MAb could well recognize ICOS molecule expressed on activated T cells and induce the activation as well as proliferation of T cells prestimulated by anti-human CD3 MAb. Furthermore, we found that MAb 2C7 could induce the growth arrest of XG2 cells, a human multiple myeloma cell line, which abnormally expressed ICOS molecule, and led to its apoptosis after 48 h of treatment. This functional anti-human ICOS MAb provides a valuable tool for further study of biological functions as well as signal transduction of ICOS/GL50.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / isolation & purification
  • Antibodies, Monoclonal / pharmacology*
  • Antibody Specificity
  • Antigens, Differentiation, T-Lymphocyte / chemistry
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • Apoptosis / drug effects
  • Cell Line
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cytokines / biosynthesis
  • Epitopes / immunology
  • Humans
  • Inducible T-Cell Co-Stimulator Protein
  • Lymphocyte Activation / drug effects*
  • Mice
  • Multiple Myeloma / pathology*
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Cytokines
  • Epitopes
  • ICOS protein, human
  • Icos protein, mouse
  • Inducible T-Cell Co-Stimulator Protein