Glutamate receptor-interacting protein 1 protein binds to the microtubule-associated protein

Biosci Biotechnol Biochem. 2004 Aug;68(8):1808-10. doi: 10.1271/bbb.68.1808.

Abstract

To identify the interaction proteins for the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor subunit glutamate receptor-interacting protein 1 (GRIP1), GRIP1 interactions with microtubule-associated protein (MAP)-1B light chain (LC) were investigated. GRIP1 interacts with MAP-1A and MAP-1B in the yeast two-hybrid assay, as is indicated also by glutathione S-transferase (GST) pull-down and coimmunoprecipitation with MAP-1B LC antibody in brain fractions. These results suggest a novel mechanism for localizing AMPA receptors to synaptic sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Brain / metabolism*
  • Carrier Proteins / metabolism*
  • Mice
  • Microtubule-Associated Proteins / metabolism*
  • Nerve Tissue Proteins / metabolism*
  • Protein Binding
  • Two-Hybrid System Techniques
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Grip1 protein, mouse
  • Microtubule-Associated Proteins
  • Nerve Tissue Proteins
  • microtubule-associated protein 1B
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid