Abstract
To identify the interaction proteins for the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor subunit glutamate receptor-interacting protein 1 (GRIP1), GRIP1 interactions with microtubule-associated protein (MAP)-1B light chain (LC) were investigated. GRIP1 interacts with MAP-1A and MAP-1B in the yeast two-hybrid assay, as is indicated also by glutathione S-transferase (GST) pull-down and coimmunoprecipitation with MAP-1B LC antibody in brain fractions. These results suggest a novel mechanism for localizing AMPA receptors to synaptic sites.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Brain / metabolism*
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Carrier Proteins / metabolism*
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Mice
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Microtubule-Associated Proteins / metabolism*
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Nerve Tissue Proteins / metabolism*
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Protein Binding
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Two-Hybrid System Techniques
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Grip1 protein, mouse
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Microtubule-Associated Proteins
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Nerve Tissue Proteins
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microtubule-associated protein 1B
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid