Structure-activity relationship for FR901464: a versatile method for the conversion and preparation of biologically active biotinylated probes

Hajime Motoyoshi; Masato Horigome; Ken Ishigami; Tatsuhiko Yoshida; Sueharu Horinouchi; Minoru Yoshida; Hidenori Watanabe; Takeshi Kitahara

doi:10.1271/bbb.68.2178

Structure-activity relationship for FR901464: a versatile method for the conversion and preparation of biologically active biotinylated probes

Biosci Biotechnol Biochem. 2004 Oct;68(10):2178-82. doi: 10.1271/bbb.68.2178.

Authors

Hajime Motoyoshi¹, Masato Horigome, Ken Ishigami, Tatsuhiko Yoshida, Sueharu Horinouchi, Minoru Yoshida, Hidenori Watanabe, Takeshi Kitahara

Affiliation

¹ Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

PMID: 15502365
DOI: 10.1271/bbb.68.2178

Abstract

The structure-activity relationship for FR901464, a potent cell-cycle inhibitor, was examined by synthesizing its analogs. A versatile method for converting FR901464 was devised. This method made it possible to synthesize biologically active FR901464-biotin conjugates which could be used to isolate the binding proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotin / chemistry*
Biotinylation*
Cell Cycle / drug effects*
Pyrans / analysis
Pyrans / chemical synthesis
Pyrans / chemistry*
Pyrans / pharmacology
Spiro Compounds / analysis
Spiro Compounds / chemical synthesis
Spiro Compounds / chemistry*
Spiro Compounds / pharmacology
Structure-Activity Relationship

Substances

FR 901464
Pyrans
Spiro Compounds
Biotin