5'SAGE: 5'-end Serial Analysis of Gene Expression database

Yasuhiro Kasai; Shin-ichi Hashimoto; Tomoyuki Yamada; Jun Sese; Sumio Sugano; Kouji Matsushima; Shinichi Morishita

doi:10.1093/nar/gki085

5'SAGE: 5'-end Serial Analysis of Gene Expression database

Nucleic Acids Res. 2005 Jan 1;33(Database issue):D550-2. doi: 10.1093/nar/gki085.

Authors

Yasuhiro Kasai¹, Shin-ichi Hashimoto, Tomoyuki Yamada, Jun Sese, Sumio Sugano, Kouji Matsushima, Shinichi Morishita

Affiliation

¹ Department of Computational Biology, Graduate School of Frontier Science, The University of Tokyo, 5-1-5 Kashinoha, Kashiwa City, Chiba 277-8562, Japan.

Abstract

To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5' end Serial Analysis of Gene Expression (SAGE) was developed recently, which makes it possible to collect a large amount of start site information, and subsequently, we have established a related database server called 5'SAGE. This database displays the observed frequencies of individual 5' end SAGE tags and previously unknown transcription start sites in the promoter regions, introns and intergenic regions of known genes. 5'SAGE will be useful for analyzing promoter regions and start site variation in different tissues, and is freely available at http://5sage.gi.k.u-tokyo.ac.jp/.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5' Flanking Region
Cell Line
Databases, Nucleic Acid*
Gene Expression Profiling*
Genome, Human
Humans
Sequence Tagged Sites
Transcription Initiation Site*