Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays

Kazunori Shimizu; Shuhei Hayashi; Takeshi Kako; Maiko Suzuki; Norihiko Tsukagoshi; Noriyuki Doukyu; Takeshi Kobayashi; Hiroyuki Honda

doi:10.1128/AEM.71.2.1093-1096.2005

Discovery of glpC, an organic solvent tolerance-related gene in Escherichia coli, using gene expression profiles from DNA microarrays

Appl Environ Microbiol. 2005 Feb;71(2):1093-6. doi: 10.1128/AEM.71.2.1093-1096.2005.

Authors

Kazunori Shimizu¹, Shuhei Hayashi, Takeshi Kako, Maiko Suzuki, Norihiko Tsukagoshi, Noriyuki Doukyu, Takeshi Kobayashi, Hiroyuki Honda

Affiliation

¹ Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

Abstract

Gene expression profiles were collected from Escherichia coli strains (OST3410, TK33, and TK31) before and after exposure to organic solvents, and the six genes that showed higher gene expression were selected. Among these genes, glpC encoding the anaerobic glycerol-3-phosphate dehydrogenase subunit C remarkably increased the organic solvent tolerance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclohexanes / pharmacology
Escherichia coli K12 / drug effects*
Escherichia coli K12 / genetics*
Escherichia coli K12 / growth & development
Escherichia coli K12 / metabolism
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Gene Expression Profiling*
Gene Expression Regulation, Bacterial*
Glycerolphosphate Dehydrogenase / genetics*
Glycerolphosphate Dehydrogenase / metabolism
Oligonucleotide Array Sequence Analysis / methods
Solvents / pharmacology*
Xylenes / pharmacology

Substances

Cyclohexanes
Escherichia coli Proteins
Solvents
Xylenes
Glycerolphosphate Dehydrogenase