Using genome-wide transcriptional profiling to elucidate small-molecule mechanism

Rebecca A Butcher; Stuart L Schreiber

doi:10.1016/j.cbpa.2004.10.009

Using genome-wide transcriptional profiling to elucidate small-molecule mechanism

Curr Opin Chem Biol. 2005 Feb;9(1):25-30. doi: 10.1016/j.cbpa.2004.10.009.

Authors

Rebecca A Butcher¹, Stuart L Schreiber

Affiliation

¹ Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.

PMID: 15701449
DOI: 10.1016/j.cbpa.2004.10.009

Abstract

Transcriptional profiling with DNA microarrays can be used to measure the genome-wide transcriptional response to small molecules. Recent progress in the analysis of gene-expression data has relied on the generation of databases of profiles documenting the transcriptional effects of various compound treatments and genetic perturbations. A positive correlation between the transcriptional response induced by a novel small molecule and a database profile can provide insight into the molecule's mechanism. Transcriptional profiling can also be used to assess a small molecule's specificity for its target and to facilitate analysis of pathways downstream of the target.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Review

MeSH terms

Gene Expression Profiling*
Genomics
Humans
Oligonucleotide Array Sequence Analysis / methods*
Transcription, Genetic* / drug effects