Molecular profiling of Bacteroides spp. in human feces by PCR-temperature gradient gel electrophoresis

Xiaoyan Pang; Dezhong Ding; Guifang Wei; Meiling Zhang; Linghua Wang; Liping Zhao

doi:10.1016/j.mimet.2004.12.012

Molecular profiling of Bacteroides spp. in human feces by PCR-temperature gradient gel electrophoresis

J Microbiol Methods. 2005 Jun;61(3):413-7. doi: 10.1016/j.mimet.2004.12.012. Epub 2005 Jan 22.

Authors

Xiaoyan Pang¹, Dezhong Ding, Guifang Wei, Meiling Zhang, Linghua Wang, Liping Zhao

Affiliation

¹ Laboratory of Molecular Microbial Ecology and Ecogenomics, Department of Bioscience and Biotechnology, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

PMID: 15767017
DOI: 10.1016/j.mimet.2004.12.012

Abstract

A group-specific PCR-based temperature gradient gel electrophoresis (TGGE) method was developed to study the population composition of genus Bacteroides in human gut. Highly reproducible and well-separated bands in TGGE fingerprints of ten unrelated human fecal samples showed complex and host-specific Bacteroides species composition. Dynamic monitoring over 22 months of samples from one healthy 10-year-old boy indicated a relatively stable population profile of Bacteroides. The species identity of each band in TGGE gel of this boy was also resolved via comigration analysis of sequenced inserts in a Bacteroides group-specific clone library. This work provides a rapid and effective technique for analyzing the species composition of Bacteroides in human gut.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques
Bacteroides / classification
Bacteroides / genetics*
Bacteroides / isolation & purification*
Base Sequence
Child
DNA, Bacterial / genetics
DNA, Bacterial / isolation & purification
Electrophoresis, Polyacrylamide Gel / methods
Feces / microbiology*
Humans
Male
Polymerase Chain Reaction / methods*
Species Specificity

Substances

DNA, Bacterial