Abstract
The genome of Arabidopsis thaliana contains 13 myosin XI isoforms. Here we prepared a specific antibody against a peptide that mimics a unique C-terminal region from the myosin XI isoform, MYA2. The resulting antibody was used to demonstrate that MYA2 in Arabidopsis protein extracts co-sedimented with actin filaments and dissociated from the filaments with ATP treatment. Immunolocalization studies showed that MYA2 co-localized predominantly with actin filaments in clustered punctuate dots in leaf epidermal cells, root hair cells and suspension-cultured cells. In a transgenic plant in which peroxisomes are labeled with green fluorescent protein, some MYA2 signals were localized on peroxisomes in an actin-dependent manner. We propose that the peroxisome is one of the cargos translocated by MYA2 on actin filaments.
MeSH terms
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Actin Cytoskeleton / metabolism
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Amino Acid Sequence
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Molecular Sequence Data
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Mutation / genetics
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Myosin Heavy Chains / chemistry
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Myosin Heavy Chains / genetics
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Myosin Heavy Chains / metabolism*
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Myosins / chemistry
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Myosins / genetics
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Myosins / metabolism*
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Peroxisomes / genetics
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Peroxisomes / metabolism*
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Phenotype
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Plant Epidermis / cytology
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Plant Epidermis / metabolism
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Plant Leaves / cytology
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Plant Leaves / metabolism
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Plant Roots / cytology
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Plant Roots / metabolism
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Protein Structure, Tertiary
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Protein Transport / physiology
Substances
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Arabidopsis Proteins
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MYA2 protein, Arabidopsis
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Protein Isoforms
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Myosin Heavy Chains
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Myosins