Abstract
Both mammary gland development and mammary carcinogenesis involve extensive remodeling of the mammary gland extracellular matrix. The expression of four membrane-type matrix metalloproteinases (MT-MMPs) with matrix remodeling potential in development and tumorigenesis was evaluated by in-situ hybridization on mouse mammary gland sections. MT1-MMP and MT3-MMP were found in the mammary stroma mainly around epithelial structures in both developing and mature mammary gland. In contrast, MT2-MMP was found exclusively in the mammary epithelium. Lactating gland expressed none of the examined MT-MMPs. Mammary gland tumors expressed MT1-MMP, MT2-MMP, and MT3-MMP while MT4-MMP was not expressed in any developmental or cancerous stage analyzed here. Our results suggest that MT1-MMP, MT2-MMP, and MT3-MMP may be involved in remodeling of both the normal and diseased mammary gland either directly or indirectly by activation of other MMPs.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aging / physiology
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Animals
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Female
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Gene Expression Profiling*
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Gene Expression Regulation, Enzymologic / genetics*
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Health
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Lactation / genetics
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Lactation / metabolism
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Mammary Glands, Animal / cytology
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Mammary Glands, Animal / enzymology*
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Mammary Glands, Animal / pathology
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Mammary Neoplasms, Animal / enzymology*
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Mammary Neoplasms, Animal / genetics
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Mammary Neoplasms, Animal / pathology
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Matrix Metalloproteinase 14
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Matrix Metalloproteinase 15
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Matrix Metalloproteinase 16
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Matrix Metalloproteinases, Membrane-Associated
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Metalloendopeptidases / chemistry*
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Metalloendopeptidases / genetics
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Metalloendopeptidases / metabolism*
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Metallothionein 3
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Mice
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Pregnancy
Substances
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Metallothionein 3
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Mmp14 protein, mouse
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Mmp15 protein, mouse
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Mmp16 protein, mouse
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Mt3 protein, mouse
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Matrix Metalloproteinase 15
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Matrix Metalloproteinase 16
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Matrix Metalloproteinases, Membrane-Associated
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Metalloendopeptidases
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Matrix Metalloproteinase 14