Inclusion of a photosensitizer in liposomes formed by DMPC/gemini surfactant: correlation between physicochemical and biological features of the complexes

J Med Chem. 2005 Jul 28;48(15):4882-91. doi: 10.1021/jm050182d.

Abstract

Mixed cationic liposomes composed by different ratios of dimyristoyl-sn-glycero-phosphatidylcoline (DMPC) and a cationic gemini surfactant have been studied by various physicochemical tools as vehicles for m-tetrahydroxyphenylchlorin (m-THPC), a photosensitizer used in photodynamic therapy. Entrapment and location of m-THPC within the lipid double layer have been evaluated by different techniques and the new formulations have been tested on a stabilized cell line from a human colon tumor, COLO206. A correlation between the physicochemical features of formulations and their efficiency as photosensitizers vector was found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cations
  • Cell Line, Tumor
  • Dimyristoylphosphatidylcholine / chemistry*
  • Fluorescence
  • Humans
  • Light
  • Liposomes / chemistry*
  • Mesoporphyrins / administration & dosage
  • Mesoporphyrins / chemistry
  • Mesoporphyrins / pharmacology
  • Microscopy, Confocal
  • Microscopy, Electron, Transmission
  • Photochemotherapy
  • Photosensitizing Agents / administration & dosage
  • Photosensitizing Agents / chemistry*
  • Photosensitizing Agents / pharmacokinetics*
  • Quaternary Ammonium Compounds / chemistry*
  • Scattering, Radiation
  • Succinates / chemistry*
  • Surface-Active Agents / chemistry*
  • Transition Temperature

Substances

  • 2,3-bis((dimethyl(pentadecyl)ammonio)methyl)succinic acid
  • Cations
  • Liposomes
  • Mesoporphyrins
  • Photosensitizing Agents
  • Quaternary Ammonium Compounds
  • Succinates
  • Surface-Active Agents
  • temoporfin
  • Dimyristoylphosphatidylcholine