Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs

Bioorg Med Chem Lett. 2006 Feb;16(3):521-4. doi: 10.1016/j.bmcl.2005.10.061. Epub 2005 Nov 11.

Abstract

A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Membrane / metabolism
  • Drug Design
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Peptides / metabolism*
  • Peptides, Cyclic / chemical synthesis
  • Peptides, Cyclic / metabolism*
  • Protein Conformation
  • Proteins / chemistry
  • Proteins / metabolism
  • Receptors, G-Protein-Coupled / chemistry
  • Receptors, G-Protein-Coupled / metabolism*
  • Receptors, Vasopressin / chemistry
  • Receptors, Vasopressin / metabolism
  • Sepharose / chemistry*

Substances

  • Peptides
  • Peptides, Cyclic
  • Proteins
  • Receptors, G-Protein-Coupled
  • Receptors, Vasopressin
  • Sepharose