Structural studies of the core region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide

Zhan Wang; Jianjun Li; Evgeny Vinogradov; Eleonora Altman

doi:10.1016/j.carres.2005.10.017

Structural studies of the core region of Aeromonas salmonicida subsp. salmonicida lipopolysaccharide

Carbohydr Res. 2006 Jan 16;341(1):109-17. doi: 10.1016/j.carres.2005.10.017. Epub 2005 Nov 17.

Authors

Zhan Wang¹, Jianjun Li, Evgeny Vinogradov, Eleonora Altman

Affiliation

¹ Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6.

PMID: 16297894
DOI: 10.1016/j.carres.2005.10.017

Abstract

The core oligosaccharide structure of the in vivo derived rough phenotype of Aeromonas salmonicida subsp. salmonicida was investigated by a combination of compositional, methylation, CE-MS and one- and two-dimensional NMR analyses and established as the following: [carbohydrate: see text] where R=alpha-D-Galp-(1-->4)-beta-D-GalpNAc-(1--> or alpha-D-Galp-(1--> (approx. ratio 4:3). Comparative CE-MS analysis of A. salmonicida subsp. salmonicida core oligosaccharides from strains A449, 80204-1 and an in vivo rough isolate confirmed that the structure of the core oligosaccharide was conserved among different isolates of A. salmonicida.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas salmonicida / chemistry*
Carbohydrate Sequence
Electrophoresis, Capillary
Electrophoresis, Polyacrylamide Gel
Lipopolysaccharides / chemistry*
Mass Spectrometry
Methylation
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular

Substances

Lipopolysaccharides