Two strains of Bacillus sp. F2 and F6 could produce bioflocculants with high flocculating effects by pure culture and mixed culture. Many kinds of carbon sources could be utilized. Organic compound which molecular weight under 200 such as saccharide, alcoholic aldehyde, ester and organic acid were favorable for producing bioflocculant. And some low-cost biomass scrap could be used as good carbon sources for industrialized production of bioflocculants, such as waste beet molasses, cellulose's fermentation residue and sewage of hydrogen producing reactor.