The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus

Cell. 1975 May;5(1):51-8. doi: 10.1016/0092-8674(75)90091-4.

Abstract

The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosphated of GTP and alpha phosphate of ATP are incorporated into the blocked 5' terminal structure.

MeSH terms

  • Adenosine / analysis
  • Adenosine Triphosphate / metabolism
  • Alkaline Phosphatase / metabolism
  • Cell-Free System
  • Chromatography, DEAE-Cellulose
  • Chromatography, Paper
  • DNA-Directed RNA Polymerases / metabolism
  • Diphosphates / analysis
  • Electrophoresis, Paper
  • Guanosine / analysis
  • Guanosine Triphosphate / metabolism
  • Methylation
  • Oligonucleotides / analysis
  • Phosphoric Diester Hydrolases / metabolism
  • Pyrophosphatases / metabolism
  • RNA, Messenger / analysis*
  • RNA, Messenger / biosynthesis
  • RNA, Viral / analysis*
  • RNA, Viral / biosynthesis
  • Ribonucleases / metabolism
  • S-Adenosylmethionine / metabolism
  • Vesicular stomatitis Indiana virus / analysis*
  • Vesicular stomatitis Indiana virus / enzymology

Substances

  • Diphosphates
  • Oligonucleotides
  • RNA, Messenger
  • RNA, Viral
  • Guanosine
  • S-Adenosylmethionine
  • Guanosine Triphosphate
  • Adenosine Triphosphate
  • DNA-Directed RNA Polymerases
  • Ribonucleases
  • Alkaline Phosphatase
  • Phosphoric Diester Hydrolases
  • Pyrophosphatases
  • Adenosine