Delivery of short hairpin RNAs--triggers of gene silencing--into mouse embryonic stem cells

Nat Methods. 2006 May;3(5):397-400. doi: 10.1038/nmeth0506-397.

Authors

Christoph Schaniel¹, Feng Li, Xenia L Schafer, Troy Moore, Ihor R Lemischka, Patrick J Paddison

Affiliation

¹ Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

PMID: 16628211
DOI: 10.1038/nmeth0506-397

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Embryo, Mammalian / cytology
Embryo, Mammalian / drug effects*
Embryo, Mammalian / metabolism
Gene Silencing / drug effects*
Gene Silencing / physiology
Gene Transfer Techniques
Genes, Reporter / genetics
Genetic Engineering / methods*
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / metabolism
Mice
RNA, Small Interfering / administration & dosage
RNA, Small Interfering / metabolism
RNA, Small Interfering / pharmacology*
Stem Cells / cytology
Stem Cells / drug effects*
Stem Cells / metabolism

Substances

RNA, Small Interfering
Green Fluorescent Proteins