Abstract
A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60,771 Da.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Bacterial
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Genes, Bacterial
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Histidine / metabolism
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Molecular Sequence Data
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Multigene Family
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Polymerase Chain Reaction
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Pseudomonas / enzymology
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Pseudomonas / genetics*
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Urocanate Hydratase / genetics*
Substances
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DNA, Bacterial
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Histidine
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Urocanate Hydratase
Associated data
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GENBANK/M95211
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GENBANK/X58483
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GENBANK/X58797
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GENBANK/X58798
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GENBANK/X58799
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GENBANK/X58800
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GENBANK/X58801
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GENBANK/X58802
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GENBANK/X58803
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GENBANK/X62841