Abstract
In rat brain cortex synaptosomes superfused with Mg(2+)-free solution containing glycine, [3H]noradrenaline (3H-NA) release evoked by NMDA was abolished by omission of glycine or Ca2+ and inhibited by Mg2+, the competitive and noncompetitive NMDA receptor antagonists CGP 37849 and dizocilpine (MK-801), respectively, as well as by ethanol, but was not affected by tetrodotoxin. The 3H-NA release evoked by L-glutamate was also competitively inhibited by CGP 37849. In conclusion, presynaptic NMDA receptors mediate stimulation of NA release. Ethanol probably acts at this receptor system.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2-Amino-5-phosphonovalerate / analogs & derivatives
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2-Amino-5-phosphonovalerate / pharmacology
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Animals
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Calcium / pharmacology
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Cerebral Cortex / metabolism*
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Dizocilpine Maleate / pharmacology
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Glutamates / pharmacology
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Glutamic Acid
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Glycine / pharmacology
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Kinetics
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Magnesium / pharmacology
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Male
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N-Methylaspartate / pharmacology*
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Norepinephrine / metabolism*
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Rats
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Rats, Inbred Strains
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Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
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Receptors, N-Methyl-D-Aspartate / drug effects
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Receptors, N-Methyl-D-Aspartate / physiology*
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Synaptosomes / drug effects
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Synaptosomes / metabolism*
Substances
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Glutamates
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Receptors, N-Methyl-D-Aspartate
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2-amino-4-methyl-5-phosphono-3-pentenoic acid
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Glutamic Acid
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N-Methylaspartate
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Dizocilpine Maleate
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2-Amino-5-phosphonovalerate
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Magnesium
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Calcium
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Glycine
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Norepinephrine