Abstract
Small, reversibly biotinylated RNAs as described here are versatile ligands for affinity chromatography of RNA-binding components. These RNAs can be attached to a solid support by binding to avidin and used as ligands, or they may be hybridized to another RNA which acts as the ligand. The incorporation of a disulfide bond in the linker arm connecting biotin to the RNA makes it possible to dissociate the RNA from avidin under mild conditions. Our results regarding the binding and elution of the biotinylated RNA may be applied to other, reversibly biotinylated molecules.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Actins / genetics
-
Base Sequence
-
Biotin
-
Chromatography, Affinity / methods
-
Exons
-
Indicators and Reagents
-
Molecular Sequence Data
-
Nucleic Acid Conformation
-
RNA / isolation & purification*
-
RNA Precursors / isolation & purification*
-
RNA Splicing
-
RNA, Fungal / isolation & purification*
-
RNA, Messenger / genetics
-
Ribonucleoproteins / isolation & purification
-
Saccharomyces cerevisiae / genetics
Substances
-
Actins
-
Indicators and Reagents
-
RNA Precursors
-
RNA, Fungal
-
RNA, Messenger
-
Ribonucleoproteins
-
RNA
-
Biotin