Adhesion of human cancer cells to vascular endothelium mediated by a carbohydrate antigen, sialyl Lewis A

Biochem Biophys Res Commun. 1991 Sep 16;179(2):713-9. doi: 10.1016/0006-291x(91)91875-d.

Abstract

Recently the lectin-like domain on ELAM-1 (endothelial leukocyte adhesion molecule-1) was shown to recognize a carbohydrate antigen, sialyl Lewis x. In this paper we demonstrate, by a series of inhibition experiments utilizing specific monoclonal antibodies and pure glycolipid preparations, that the sialyl Lewis a antigen serves as a specific ligand for ELAM-1 as well as sialyl Lewis x and plays a significant role in the ELAM-1-mediated binding of human cancer cells to activated endothelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, Tumor-Associated, Carbohydrate / metabolism*
  • CA-19-9 Antigen
  • Carbohydrate Sequence
  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism
  • Drug Carriers
  • E-Selectin
  • Endothelium, Vascular / metabolism*
  • Gangliosides / metabolism*
  • Humans
  • Interleukin-1 / metabolism
  • Lewis Blood Group Antigens*
  • Lewis X Antigen / metabolism
  • Ligands
  • Liposomes
  • Molecular Sequence Data
  • Recombinant Proteins / metabolism
  • Tumor Cells, Cultured / metabolism

Substances

  • Antibodies, Monoclonal
  • Antigens, Tumor-Associated, Carbohydrate
  • CA-19-9 Antigen
  • Cell Adhesion Molecules
  • Drug Carriers
  • E-Selectin
  • Gangliosides
  • Interleukin-1
  • Lewis Blood Group Antigens
  • Lewis X Antigen
  • Ligands
  • Liposomes
  • Recombinant Proteins
  • sialyl Le(a) ganglioside