Abstract
Recently the lectin-like domain on ELAM-1 (endothelial leukocyte adhesion molecule-1) was shown to recognize a carbohydrate antigen, sialyl Lewis x. In this paper we demonstrate, by a series of inhibition experiments utilizing specific monoclonal antibodies and pure glycolipid preparations, that the sialyl Lewis a antigen serves as a specific ligand for ELAM-1 as well as sialyl Lewis x and plays a significant role in the ELAM-1-mediated binding of human cancer cells to activated endothelial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal
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Antigens, Tumor-Associated, Carbohydrate / metabolism*
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CA-19-9 Antigen
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Carbohydrate Sequence
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Cell Adhesion
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Cell Adhesion Molecules / metabolism
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Drug Carriers
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E-Selectin
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Endothelium, Vascular / metabolism*
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Gangliosides / metabolism*
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Humans
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Interleukin-1 / metabolism
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Lewis Blood Group Antigens*
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Lewis X Antigen / metabolism
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Ligands
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Liposomes
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Molecular Sequence Data
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Recombinant Proteins / metabolism
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Tumor Cells, Cultured / metabolism
Substances
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Antibodies, Monoclonal
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Antigens, Tumor-Associated, Carbohydrate
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CA-19-9 Antigen
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Cell Adhesion Molecules
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Drug Carriers
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E-Selectin
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Gangliosides
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Interleukin-1
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Lewis Blood Group Antigens
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Lewis X Antigen
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Ligands
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Liposomes
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Recombinant Proteins
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sialyl Le(a) ganglioside