Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis

Eur J Pediatr. 2007 Nov;166(11):1189-91. doi: 10.1007/s00431-006-0378-7. Epub 2006 Dec 20.

Authors

Alida M van Kruijssen¹, Kate E Templeton, Roos N van der Plas, H Rogier van Doorn, Eric C J Claas, Ram N Sukhai, Ed J Kuijper

Affiliation

¹ Department of Pediatrics, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands.

Abstract

The use of a multiplex respiratory real-time PCR in patients clinically suspected of pertussis increases the number of pathogens detected.

MeSH terms

Bacteria / genetics
Bacteria / isolation & purification
Bordetella pertussis / genetics
Bordetella pertussis / isolation & purification*
Child
Child, Preschool
Female
Humans
Infant
Infant, Newborn
Male
Polymerase Chain Reaction*
Sensitivity and Specificity
Viruses / genetics
Viruses / isolation & purification
Whooping Cough / diagnosis*