Localization of hepatitis C virus (HCV) antigen by immunohistochemistry on fixed-embedded liver tissue

Ital J Gastroenterol. 1990 Aug;22(4):198-9.

Abstract

An immunohistochemical method of staining HCV-related antigens in fixed-embedded liver biopsies is described. Two primary antisera were used: 1) a high titre anti-HCV human IgG separated from an anti-HCV positive serum; and 2) rabbit anti-HCV antibodies. In our experience this method proves to be reproducible and shows a good correlation with serologic results.

MeSH terms

  • Animals
  • Antigens, Viral / isolation & purification*
  • Biopsy
  • Fixatives
  • Hepacivirus / immunology*
  • Hepatitis Antibodies / administration & dosage
  • Hepatitis C / immunology
  • Hepatitis C / pathology
  • Hepatitis C Antigens
  • Humans
  • Immunohistochemistry
  • Liver / immunology
  • Liver / pathology*
  • Staining and Labeling
  • Tissue Embedding

Substances

  • Antigens, Viral
  • Fixatives
  • Hepatitis Antibodies
  • Hepatitis C Antigens