Stable expression of cytochrome P450IIIA7 cDNA in human breast cancer cell line MCF-7 and its application to cytotoxicity testing

Arch Biochem Biophys. 1992 Jan;292(1):136-40. doi: 10.1016/0003-9861(92)90061-z.

Abstract

A mammalian cell expression plasmid containing cytochrome P450IIIA7 complementary DNA was constructed. Breast cancer cells (MCF-7) were transfected with the plasmid and neomycin-resistant selection marker plasmid. We established three cell lines, termed M13, M21, and M27, which expressed the cytochrome P450IIIA7 as examined by RNA blot and immunoblot analyses. These cell lines showed 8- to 10-fold higher sensitivity against aflatoxin B1 compared to parental MCF-7 cells, suggesting that cytochromes P450IIIA7 expressed in the cells were responsible for the production of the cytotoxic metabolite of aflatoxin B1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aflatoxin B1 / toxicity*
  • Base Sequence
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / enzymology
  • Breast Neoplasms / genetics*
  • Cell Line, Transformed
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / chemistry*
  • Cytochrome P-450 Enzyme System / genetics
  • DNA, Neoplasm / chemistry*
  • Drug Screening Assays, Antitumor
  • Female
  • Genetic Vectors
  • Humans
  • Immunoblotting
  • Liver / embryology
  • Liver / enzymology
  • Molecular Sequence Data
  • Polymerase Chain Reaction

Substances

  • DNA, Neoplasm
  • Cytochrome P-450 Enzyme System
  • Aflatoxin B1