Abstract
Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Chromosomes / metabolism
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Drosophila Proteins / chemistry
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / enzymology*
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Histone Demethylases
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Histone-Lysine N-Methyltransferase / chemistry
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Histone-Lysine N-Methyltransferase / genetics*
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Histone-Lysine N-Methyltransferase / metabolism*
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Histones / metabolism*
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Larva / cytology
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Lysine / metabolism*
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Methylation
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Protein Binding
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RNA Interference
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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CHD1 protein, Drosophila
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DNA-Binding Proteins
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Drosophila Proteins
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Histones
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Transcription Factors
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Trl protein, Drosophila
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Histone Demethylases
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Lid protein, Drosophila
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Histone-Lysine N-Methyltransferase
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Lysine