The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase

Nat Struct Mol Biol. 2007 Apr;14(4):344-6. doi: 10.1038/nsmb1217. Epub 2007 Mar 11.

Abstract

Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chromosomes / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / enzymology*
  • Histone Demethylases
  • Histone-Lysine N-Methyltransferase / chemistry
  • Histone-Lysine N-Methyltransferase / genetics*
  • Histone-Lysine N-Methyltransferase / metabolism*
  • Histones / metabolism*
  • Larva / cytology
  • Lysine / metabolism*
  • Methylation
  • Protein Binding
  • RNA Interference
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • CHD1 protein, Drosophila
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Histones
  • Transcription Factors
  • Trl protein, Drosophila
  • Histone Demethylases
  • Lid protein, Drosophila
  • Histone-Lysine N-Methyltransferase
  • Lysine