Phylotype diversity of deep-sea hydrothermal vent prokaryotes trapped by 0.2- and 0.1-microm-pore-size filters

Extremophiles. 2007 Jul;11(4):637-46. doi: 10.1007/s00792-007-0070-5. Epub 2007 Mar 31.

Abstract

Eleven 16S rRNA gene clone libraries including 34 archaeal and 72 bacterial phylotypes were constructed from total 708 clones of hydrothermal vent prokaryotes trapped by 0.2- and 0.1-microm-pore-size filters. Crenarchaeota and Proteobacteria phylotypes dominated the archaeal and bacterial populations, respectively. Novel unaffiliated phylotypes occurred only in the 0.1-microm-trapped populations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaea / classification*
  • Archaea / genetics
  • Archaea / isolation & purification
  • Bacteria / classification*
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Biodiversity*
  • Crenarchaeota / classification
  • DNA, Archaeal / analysis
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Filtration / instrumentation*
  • Gene Library
  • Japan
  • Micropore Filters*
  • Phylogeny*
  • Porosity
  • Prokaryotic Cells
  • RNA, Ribosomal, 16S
  • Ribotyping
  • Seawater / microbiology*
  • Water Microbiology*

Substances

  • DNA, Archaeal
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S