Abstract
Enzymes expressed in response to vinyl chloride, ethene, and epoxyethane by Nocardioides sp. strain JS614 were identified by using a peptide mass fingerprinting (PMF) approach. PMF provided insight concerning vinyl chloride biodegradation in strain JS614 and extends the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry as a tool to enhance characterization of biodegradation pathways.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Base Sequence
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Biodegradation, Environmental
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Carcinogens, Environmental / metabolism
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Carcinogens, Environmental / toxicity
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Enzymes / biosynthesis
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Enzymes / genetics
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Enzymes / isolation & purification
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Epoxy Compounds / metabolism
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Epoxy Compounds / toxicity*
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Ethylenes / metabolism
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Ethylenes / toxicity*
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Genes, Bacterial
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Molecular Sequence Data
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Peptide Mapping
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Propionibacteriaceae / drug effects*
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Propionibacteriaceae / genetics
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Propionibacteriaceae / metabolism*
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Proteomics
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Reverse Transcriptase Polymerase Chain Reaction
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Vinyl Chloride / metabolism
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Vinyl Chloride / toxicity*
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Water Pollutants, Chemical / metabolism
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Water Pollutants, Chemical / toxicity
Substances
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Bacterial Proteins
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Carcinogens, Environmental
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DNA Primers
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DNA, Bacterial
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Enzymes
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Epoxy Compounds
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Ethylenes
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Water Pollutants, Chemical
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ethylene
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Vinyl Chloride
Associated data
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GENBANK/Q56839
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RefSeq/ZP_00655558
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RefSeq/ZP_00655559
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RefSeq/ZP_00655566
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RefSeq/ZP_00655570
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RefSeq/ZP_00655575
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RefSeq/ZP_00655584