[Valuation for usefulness of selected chromosomal markers for Bacillus anthracis identification. I. Valuation for markers SG-749, SG-450 and SG-300]

Med Dosw Mikrobiol. 2006;58(4):347-54.
[Article in Polish]

Abstract

The article presents results of valuation for B. anthracis-specificity and usefulness for its identification obtained for different chromosomal markers. In the first part of the study markers SG-749, SG-300 and SG-450 were analyzed. For the investigation RFLP-PCR and MSSCP techniques were used and different electrophoresis methods were tested. The results gave an information not only about specificity of tested markers but also about the possibility of shorten time necessary to obtain results of B. anthracis identification.

Publication types

  • Evaluation Study

MeSH terms

  • Bacillus anthracis / classification
  • Bacillus anthracis / genetics*
  • Bacillus anthracis / isolation & purification*
  • Bacillus cereus / classification
  • Bacillus cereus / isolation & purification
  • Bacillus thuringiensis / classification
  • Bacillus thuringiensis / isolation & purification
  • Bacterial Typing Techniques*
  • Chromosomes, Bacterial / genetics*
  • DNA Primers
  • DNA Probes / genetics*
  • DNA, Bacterial
  • Electrophoresis / methods
  • Genetic Markers / genetics*
  • Minisatellite Repeats / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single-Stranded Conformational
  • Species Specificity

Substances

  • DNA Primers
  • DNA Probes
  • DNA, Bacterial
  • Genetic Markers