A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin

FEBS Lett. 1991 Dec 16;295(1-3):63-6. doi: 10.1016/0014-5793(91)81385-l.

Abstract

The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aequorin / chemistry
  • Aequorin / genetics*
  • Aequorin / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Circular Dichroism
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Luminescence
  • Molecular Sequence Data
  • Proline*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Scyphozoa

Substances

  • Recombinant Proteins
  • Aequorin
  • Proline