Abstract
The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline deleted showed a small change in secondary structure. In all cases studied, the C-terminal proline was required for bioluminescence activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Aequorin / chemistry
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Aequorin / genetics*
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Aequorin / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Circular Dichroism
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Cloning, Molecular
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Escherichia coli / genetics
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Luminescence
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Molecular Sequence Data
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Proline*
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Restriction Mapping
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Scyphozoa
Substances
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Recombinant Proteins
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Aequorin
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Proline