This brief report describes cultural adaptation of the WHOQOL-100 questionnaire for Taiwan. The standard cultural adaptation procedure was used for questionnaire translation, response scale generation, and national items design. A field test was conducted on 1068 participants randomly selected from 17 hospitals all over Taiwan. Several psychometric criteria were used for selecting 12 out of the 20 national items previously proposed from three focus groups. In terms of reliability and validity, the psychometric properties of the culturally adapted WHOQOL questionnaire are good and the values are comparable with those in studies conducted in 15 countries.