Influences of parental rearing on the personality traits of healthy subjects were studied in 323 Japanese volunteers. Perceived parental rearing was assessed with the use of the Parental Bonding Instrument, which consists of the factors of care and protection, whereas personality traits were assessed with the use of the Temperament and Character Inventory, which has 7 dimensions. In male subjects, all personality dimensions except for novelty seeking were influenced by parental rearing; in female subjects, only the harm avoidance (HA) and self-directedness (SD) dimensions were affected by parenting. Paternal rearing influenced 3 dimensions in male subjects and 1 dimension in female subjects, whereas maternal rearing influenced 5 dimensions in male subjects and 2 dimensions in female subjects. In male subjects, higher HA was related to higher paternal protection (P < .05), whereas in female subjects, it was related to higher maternal protection (P < .01). In male subjects, lower SD was related to higher paternal protection (P < .05) and lower maternal care (P < .01), whereas in female subjects, it was related to lower paternal care (P < .05) and higher maternal protection (P < .01). These results suggest that parental rearing influences the personality traits of healthy subjects, especially HA and SD, with sex specificity in parents and recipients.