In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography

Nat Methods. 2008 Jan;5(1):45-7. doi: 10.1038/nmeth1149. Epub 2007 Dec 9.

Abstract

We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Drosophila melanogaster / cytology*
  • Image Enhancement / methods*
  • Imaging, Three-Dimensional / methods*
  • Lighting / methods
  • Microscopy, Fluorescence / methods*
  • Pupa / cytology
  • Tomography, Optical / methods*