Abstract
We isolated and characterized a new Escherichia coli gene, htpX. The htpX gene has been localized at min 40.3 on the chromosome. We determined its transcription and translation start site. htpX expresses a 32-kDa protein from a monocistronic transcript; expression of this protein is induced by temperature upshift. htpX is expressed from a sigma 32-dependent promoter and is thus part of the heat shock regulon. Cells carrying a htpX gene disruption grow well at all temperatures and under all conditions tested and have no apparent phenotype. However, cells which overexpress a truncated form of the protein display a higher rate of degradation of puromycyl peptides.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Bacteriophage lambda
-
Base Sequence
-
Blotting, Northern
-
Blotting, Southern
-
Blotting, Western
-
Escherichia coli / genetics*
-
Escherichia coli Proteins*
-
Genes, Bacterial
-
Heat-Shock Proteins / genetics*
-
Heat-Shock Proteins / physiology
-
Hot Temperature
-
Lysogeny / drug effects
-
Metalloproteases
-
Molecular Sequence Data
-
Plasmids
-
Promoter Regions, Genetic / genetics
-
Proteins / metabolism
-
RNA, Messenger / analysis
-
Restriction Mapping
Substances
-
Escherichia coli Proteins
-
Heat-Shock Proteins
-
Proteins
-
RNA, Messenger
-
HtpX protein, E coli
-
Metalloproteases