Abstract
Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(D,L-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9% SIINFEKL/H-2K(b) tetramer positive CTLs, production of IFN-gamma, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / pharmacology
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Animals
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Antigens / administration & dosage*
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Bone Marrow Cells / immunology
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CD8-Positive T-Lymphocytes / drug effects
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CD8-Positive T-Lymphocytes / immunology
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Cell Survival / drug effects
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CpG Islands
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Cytokines / metabolism
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Dendritic Cells / immunology
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Drug Compounding
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Excipients
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Flow Cytometry
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Immunity, Cellular / drug effects
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Immunity, Cellular / immunology*
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Interferon Inducers / pharmacology
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Lactic Acid
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Ligands
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Mice
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Mice, Inbred C57BL
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Microspheres*
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Myosin Heavy Chains / metabolism
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Oligonucleotides / pharmacology
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Poly I-C
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Polyglycolic Acid
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Polylactic Acid-Polyglycolic Acid Copolymer
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T-Lymphocytes, Cytotoxic / immunology*
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Toll-Like Receptors / drug effects*
Substances
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Adjuvants, Immunologic
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Antigens
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Cytokines
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Excipients
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Interferon Inducers
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Ligands
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Oligonucleotides
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Toll-Like Receptors
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polyglycolic Acid
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Lactic Acid
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Myosin Heavy Chains
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Poly I-C