Improved performance of enzyme-linked immunosorbent assays and the effect of human immunodeficiency virus coinfection on the serologic detection of herpes simplex virus type 2 in Rakai, Uganda

Clin Vaccine Immunol. 2008 May;15(5):888-90. doi: 10.1128/CVI.00453-07. Epub 2008 Mar 5.

Abstract

Ugandan subjects (820) were tested by Focus HerpeSelect enzyme-linked immunosorbent assay (ELISA), Kalon herpes simplex virus type 2 ELISA, and BioKit rapid test, and the results were compared to those of Western blotting. Higher-than-standard-index cutoff values gave optimal sensitivity and specificity. Kalon ELISA was the optimal assay when an index value of 1.5 was used (sensitivity, 91.7%; specificity, 92.4%).

Publication types

  • Randomized Controlled Trial
  • Research Support, N.I.H., Intramural

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Viral / blood*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • HIV / immunology
  • HIV / isolation & purification*
  • HIV Seropositivity / complications
  • Herpes Genitalis / diagnosis*
  • Herpes Genitalis / epidemiology
  • Herpesvirus 2, Human / immunology*
  • Humans
  • Male
  • Reagent Kits, Diagnostic*
  • Sensitivity and Specificity
  • Uganda / epidemiology

Substances

  • Antibodies, Viral
  • Reagent Kits, Diagnostic