Abstract
A simple and efficient method for C-terminal sequencing of proteins has long been pursued because it would provide substantial information for identifying the covalent structure, including post-translational modifications. However, there are still significant impediments to both direct sequencing from C termini of proteins and specific isolation of C-terminal peptides from proteins. We describe here a highly successful, de novo C-terminal sequencing method of proteins by employing succinimidyloxycarbonylmethyl tris (2,4,6-trimethoxyphenyl) phosphonium bromide and mass spectrometry.
MeSH terms
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Animals
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Caseins / analysis*
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Cattle
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Chickens
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Cytochromes c / analysis*
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Horses
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Indicators and Reagents
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Muramidase / analysis*
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Organophosphorus Compounds / metabolism
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Ovalbumin / analysis*
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Peptide Fragments / analysis*
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Peptide Mapping
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Protein Processing, Post-Translational
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Recombinant Proteins / analysis
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Spectrometry, Mass, Electrospray Ionization
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Substances
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Caseins
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Indicators and Reagents
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Organophosphorus Compounds
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Peptide Fragments
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Recombinant Proteins
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tris(trimethoxyphenyl)phosphonium acetyl
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Ovalbumin
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Cytochromes c
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Muramidase