Regulated import and degradation of a cytosolic protein in the yeast vacuole

Nature. 1991 Mar 28;350(6316):313-8. doi: 10.1038/350313a0.

Abstract

The key regulatory enzyme in gluconeogenesis, fructose 1,6-bisphosphatase (FBPase) is subject to glucose-stimulated proteolytic degradation in Saccharomyces cerevisiae. This process involves the regulated transfer of FBPase directly from the cytosol into the vacuole or a vacuole-related organelle. Glucose may regulate the production of an FBPase receptor or import factor that is transported to the vacuole through the secretory pathway.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cytosol / metabolism
  • Endopeptidases / metabolism*
  • Fructose-Bisphosphatase / isolation & purification
  • Fructose-Bisphosphatase / metabolism*
  • Kinetics
  • Models, Biological
  • Molecular Weight
  • Mutation
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Vacuoles / metabolism*
  • Vacuoles / ultrastructure

Substances

  • Fructose-Bisphosphatase
  • Endopeptidases