Multiplex fast real-time PCR for quantitative detection and identification of cos- and pac-type Streptococcus thermophilus bacteriophages

Appl Environ Microbiol. 2008 Aug;74(15):4779-81. doi: 10.1128/AEM.00295-08. Epub 2008 Jun 6.

Abstract

The fermentation of milk by Streptococcus thermophilus is a widespread industrial process that is susceptible to bacteriophage attack. In this work, a preventive fast real-time PCR method for the detection, quantification, and identification of types of S. thermophilus phages in 30 min is described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Restriction Mapping
  • Sensitivity and Specificity
  • Streptococcus Phages / classification
  • Streptococcus Phages / genetics*
  • Streptococcus thermophilus / virology*

Substances

  • DNA Primers