Peptide mass fingerprinting using isotopically encoded photo-crosslinking amino acids

Bryan J Wilkins; Kelly A Daggett; T Ashton Cropp

doi:10.1039/b801512k

Peptide mass fingerprinting using isotopically encoded photo-crosslinking amino acids

Mol Biosyst. 2008 Sep;4(9):934-6. doi: 10.1039/b801512k. Epub 2008 Jul 17.

Authors

Bryan J Wilkins¹, Kelly A Daggett, T Ashton Cropp

Affiliation

¹ Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.

PMID: 18704231
DOI: 10.1039/b801512k

Abstract

When isotopically labelled photo-crosslinking amino acids are site-specifically incorporated into proteins, in combination with the corresponding non-labeled analogue, cross-linked tryptic peptides are easily identified in mass spectra via characteristic "doublet" patterns.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acids / chemistry*
Amino Acids / metabolism
Cross-Linking Reagents / chemistry
Isotope Labeling
Mass Spectrometry
Models, Molecular
Molecular Weight
Peptide Fragments / chemistry*
Peptide Fragments / metabolism
Peptide Mapping / methods*

Substances

Amino Acids
Cross-Linking Reagents
Peptide Fragments