Effect of magnesium ions on the inhibition of S-adenosylmethionine decarboxylase from Escherichia coli by [2-(amino-oxy)ethyl](5'-deoxyadenosin-5'-yl)(methyl)sulphonium

E L Weitkamp; H B Dixon; A R Khomutov; R M Khomutov

doi:10.1042/bj2770643

Effect of magnesium ions on the inhibition of S-adenosylmethionine decarboxylase from Escherichia coli by [2-(amino-oxy)ethyl](5'-deoxyadenosin-5'-yl)(methyl)sulphonium

Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):643-5. doi: 10.1042/bj2770643.

Authors

E L Weitkamp¹, H B Dixon, A R Khomutov, R M Khomutov

Affiliation

¹ Department of Biochemistry, University of Cambridge, U.K.

Abstract

[2-(Amino-oxy)ethyl](5'-deoxyadenosin-5'-yl)(methyl)sulphonium+ ++, the amino-oxy analogue of decarboxylated S-adenosylmethionine, is a potent irreversible inhibitor of Escherichia coli S-adenosylmethionine decarboxylase [Khomutov, Zavalova, Syrku, Artamonova & Khomutov (1983) Bioorg. Khim. 9, 130-131; Artamonova, Zavalova, Khomutov & Khomutov (1986) Bioorg. Khim. 12, 206-212]. We have shown that Mg2+ ions are required for the irreversible inhibition of the decarboxylase, and that S-adenosylmethionine protects against this inhibition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosylmethionine Decarboxylase / antagonists & inhibitors*
Deoxyadenosines / pharmacology*
Escherichia coli / enzymology
Magnesium / pharmacology*
Protein Binding
S-Adenosylmethionine / metabolism

Substances

Deoxyadenosines
S-Adenosylmethionine
S-(5'-deoxyadenosyl-5')-S-methylthioethylhydroxylamine
Adenosylmethionine Decarboxylase
Magnesium